Plates for thin - layer chromatography ( tlc ) with an attached layer of porous polymer monolith have been prepared and used for the separation of small molecules , peptides , and proteins 制备了涂覆有整体多孔聚合涂层的薄层色谱( tlc )板,并将其用于小分子、肽段和蛋白质的分离。
Second , we used high performance liquid chromatography ( hplc ) , thin - layer chromatography ( tlc ) , gel chromatography ( gc ) , and protein and nucleic acid analytic apparatus to evaluate f i " s purification 其次,利用高效液相色谱,蛋白核酸分析仪,薄层层析,凝胶色谱等检测手段,从各个角度入手,证明该f为单一组分。
Extract the bursin from the bursa fabricius of chichens and ducks through the ultrafilter with loooda filter . ( the bursin of chickens - cbs . the bursin of ducks - dbs ) determine the major constitution by high - performance liquid chromatography ( hplc ) and thin - layer chromatography ( tlc ) . it is proved that there is bursin ( bs ) in two extractions . caculate the quantitation of bursin in the extraction of the bursa fabricius of chickens and ducks . it can be found through tlc that the quantitation of cbs in the extraction of the bursa fabricius of chickens is 1 . 436mg / ml and the quantitation of dbs in the extraction of the bursa fabricius of ducks is 4 . 035mg / ml . the result obtained through hplcis that the quantitation of cbs is 1 . 199mg / ml and dbs is 3 . 316mg / ml 采用超滤法从正常鸡、鸭法氏囊组织中提取分子量小于1000da的物质,分别以薄层层析法和高压液相色谱法检测其主要成分,证明两种超滤液中均有法氏囊活性肽(囊素三肽, bursin , bs )的存在,分别计算鸡、鸭法氏囊提取物中的囊素三肽的含量。薄层层析方法求得鸡法氏囊提取物中的bs含量为1 . 436mg / ml ,鸭法氏囊提取物中的bs含量为4 . 035mg / ml ;高压液相色谱的方法求得鸡法氏囊提取物中的贴含量为1 . 199mg / ml ,鸭法氏囊提取物中bs含量为3 . 316mg / ml 。